| Secretary of State | James Madison, 1801 |
| Secretary of the Treasury | Samuel Dexter (Cont.) Albert Gallatin, 1801 |
| Secretary of War | Henry Dearborn, 1801 |
| Attorney General | Levi Lincoln, 1801 Robert Smith, 1805 John Breckinridge, 1805 Caesar A. Rodney, 1807 |
| Secretary of the Navy | Benjamin Stoddert (Cont.) Robert Smith, 1801 |